Do Ustad follows the journey of two estranged brothers and how fate brings them face-to-face with one another years later, in a courtroom. The story starts with Jagannath and Rajan doing odd jobs to make a livelihood after their parents’ death. When they are fired from work, Rajan resorts to stealing though his elder brother despises his habit. But the worse strikes when Rajan gets arrested on one of such jobs. Jagannath now on his own ends up taking up the same habit and becomes an expert at it. Years later, when Rajan is released from jail, he first decides to find his brother. So what happens when Rajan discovers that his brother who once despises his thieving habits is now a crime lord.